EIE_MDIPA_PBMCs

Immunomodulating activity of Pleurotus eryngii mushrooms following their in vitro fermentation by human fecal microbiota
Conclusion:
Overall, compared to baseline RPMI condition, NC-treated PBMCs exhibited small fold increases in certain populations of monocytes and dendritic cells as opposed to the PE condition.Interestingly, significant fold decrease in the percentages of non-classical and transitional monocytes (characterised by the expression of CD16 and CD14), as well as of myeloid dendritic cells (DCs), was observed in the PE-treated PBMCs.
Notes:
We investigated the immunomodulating effects of Pleurotus eryngii mushrooms, selected due to their high β-glucan content, strong lactogenic effect and potent geno-protective properties, following in vitro fermentation by fecal inocula from healthy elderly volunteers. The immunomodulating properties of the fermentation supernatants (FSs) were initially investigated in U937-derived human macrophages.The FSs immunomodulating effect on the volunteers’ peripheral blood mononuclear cells (PBMCs) was verified through the use of cytometry by time of flight (CyTOF) analysis. FCS collection for software testing