rawdata.7z

Instruction: More and more attention has been paid to the role of synonymous substitution in evolution, in which codon usage preference can affect gene expression distribution and protein structure and function. Vesicular glutamate transporter (VGLUT) consists of three isoforms, among which VGLUT3 is significantly different from other VGLUTs in functional importance, expression level, and distribution range, whose reason is still unclear. This study sought to analyze the role of codon preference in VGLUT differentiation.Method: To conduct an evolutionary analysis of the three VGLUTs, this paper uses bioinformatics research methods to analyze the coding sequences of the three VGLUTs in different species and compare the codon usage patterns. Furthermore, the differences among the three VGLUTs were analyzed by combining functional importance, expression level, distribution range, gene structure, protein relationship network, expression at specific developmental stages, and evolutionary relationship tree, and the influence of codon usage pattern was explored.Result: The results showed that the greater the VGLUT codon usage preference was, the less important the gene function was, the lower the expression specificity was, and the longer the time needed for development to maturity was. VGLUT3 has the largest interspecies evolutionary distance, VGLUT2 has the smallest interspecies evolutionary distance, VGLUT3 has a large evolutionary distance with the rest of the VGLUT, and the degree of codon usage preference of VGLUT is positively correlated with the rate of protein evolution.Conclusion: Codon usage preference is a potential factor affecting VGLUT developmental expression and protein evolution.