To investigate the treatment/amelioration effects of berberine on insulin resistance (IR) associated with polycystic ovary syndrome (PCOS).

16S amplicon and metabolomics analyses were performed on different groups of rat models treated with berberine. We used 16S amplicon analysis to demonstrate the microbiota with the quasi-likelihood (QL) F-test of the edgeR package. We performed different metabolite analysis using principal component analysis (PCA), partial least squares discrimination analysis (PLS-DA), and orthogonal partial least squares discrimination analysis (OPLS-DA). We then constructed a correlation network of different microbiotas and metabolites through relevance, and analyzed correlations between/among different microbiotas and metabolites.