Role of Fgf10 signalling in regulation of gene expression in developing mouse cortex. Role of Fgf10 signalling in regulation of gene expression in developing mouse cortex

The experiment was carried out to study the role of Fgf10 in mouse cortical development. To this end, we used paired-end RNA sequencing (RNA-seq) to compare polyadenylated RNA fractions from embryonic day 11.5 (E11.5) cortices of Fgf10-knockout mice and their wild-type littermates. Alternatively, E10.5 embryos were injected in utero with purified mouse Fgf10 protein dissolved in PBS or PBS only and polyadenylated RNA fractions from the injected corices were analyzed by paired-end RNA-seq 24 hours later.