Transcriptome analysis of the larval zebrafish brain

We applied zebrafish whole genome microarrays to identify molecular effects of suvorexant, imipramine and a orexin antagonist from natural plant. Behavioral assays were performed to analyze for correlations between altered gene expression with effects on the organism level. Central nervous system drug induced gene expression in larval zebrafish brain was measured at 3 hours after exposure of 1uM suvorexant, 1uM imipramine, 10uM 8beta-(4'-Hydroxytigloyloxy) costunolide or 100uM 8beta-(4'-Hydroxytigloyloxy) costunolide. Three independent experiments were performed.Gene expression profile of brain tissue of 10uM 8beta-(4'-Hydroxytigloyloxy) costunolide treated larva showed closest similarity with suvorexant, as assumed by behavior profile. Chemical-induced transcriptional changes were measured in 8dpf larval zebrafish. Gene expression in zebrafish brain was measured after exposure for 3hours to 1uM suvorexant, 1uM imipramine, 10uM 8beta-(4'-Hydroxytigloyloxy) costunolide or 100uM 8beta-(4'-Hydroxytigloyloxy) costunolide. Three biological replicates of 10 pooled brain were used for each treatment.