Fecal metabolomics,16S rRNA sequencing, metagenomics, metaproteomics and single-cell sequencing

Fecal samples from 35 acromegaly patients and 25 control patients (with sellar region lesions but no pituitary dysfunction) were subjected to 16S rRNA sequencing and metabolomics analysis. Subsequently, fecal microbiota from 20 randomly selected human donors (10 per group) was transplanted into 20 germ-free mice, followed by single-cell sequencing analysis of colonic tissues. The fecal samples from these 10 acromegaly patients and 10 control subjects were then analyzed using metagenomics and metaproteomics. Additionally, intestinal polyp samples from 6 acromegaly patients and 6 control patients were selected for proteomic analysis. Finally, target bacteria were transplanted into germ-free mice, and single-cell sequencing analysis was performed on the colonic tissues of these mice.