diaPASEF optimization for peptide profiling of hydrolyzed infant formula by microLC-timsTOF Pro

This study optimized identification rate and reproducibility of an untargeted diaPASEF approach for quantitative peptide profiling of hydrolyzed infant formula. For this, the impact of variable window widths as well as major acquisition parameters, such as the precursor coverage, number of diaPASEF scans, number of ion mobility windows and the resulting cycle time were evaluated.