Highly accurate Skin-Specific methylome analysis algorithm as a platform to screen and validate therapeutics for healthy aging [DS4]

In the present study, we developed a highly accurate Skin-Specific DNAm age predictor, using DNAm data obtained from 508 human skin samples. Based on the analysis of 2,266 CpG sites, we accurately calculated the DNAm age of cultured skin cells, and human skin biopsies. Age estimation was sensitive to the biological age of the donor, cell passage, skin disease status, as well as treatment with senotherapeutic drugs. Analyze changes in the methylation profile of human progeria dermal fibroblasts treated for 72 hours with 1.25uM ABT-263, 5uM ABT-263, 100nM Rapamycin compared to no treatment. Sample 1: human progeria dermal fibroblast, no treatment, in triplicate Sample 2: human progeria dermal fibroblast, treated with 1.25uM ABT-263, in triplicate Sample 3: human progeria dermal fibroblast, treated with 5uM ABT-263, in triplicate Sample 4: human progeria dermal fibroblast, treated with 100nM Rapamycin, in triplicate