pp71 status, HCMV serology and IF analysis of primary GBM samples.

Serology for IgG and IgM was performed and charted relative to pp71 expression status as determined by RT-PCR, TaqMan and western blot in figure 1. Flash frozen tissue sections were analyzed for the presence of pp71, SCF and CD31 by immunofluorescence (IF) in selected tumor cases, as indicated. GBM patient samples without available plasma were labeled N/A. Tumors were classified as low grade astrocytoma (LGA) or GBM by pathology, and the GBM samples were further subclassified as proneural (PN), mesenchymal (MES), or classical (CLAS) based on their gene expression signatures (data not shown and figure 6C). BC refers to a non-tumor surgical specimen from a blood clot. ND signifies not determined.