Online resources: Portuguese Pinus nigra populations: genetic diversity, structure and relationships inferred by SSR markers

<b>Online resources 1</b>: SSR database obtained by thirteen SSR markers for six populations of Pinus nigra in Portugal. The populations are representative of the species distribution in Portugal: Manteigas and Vale do Zêzere (center), Campeã and Vila Pouca de Aguiar (north) and Paredes de Coura and Caminha (northwest).<b>Online resources 2</b>: Genetic diversity statistics assayed per Portuguese P. nigra population and SSR locus. Notes: na - observed number of alleles; ne - effective number of alleles (Kimura and Crow 1964); I - Shannon’s Information Index (Lewontin 1972); h – Nei’s gene diversity index (Nei 1973); Ho – observed heterozygosity; He – expected heterozygosity (Levene 1949); the F – fixation index; and s.d. – standard deviation.<b>Online resources 3</b>: Genetic differentiation coefficient (Gst) and differentiation index of allele frequencies (Jost’s D) estimated per SSR locus, and total mean value (± standard deviation, s.d.) determined with the 13 SSR markers.<b>Online resources 4</b>: PCoA based on the pooled SSR data using the Nei’s genetic distance coefficient (Nei 1978), showing the projection of the six Portuguese P. nigra populations apart from the seven foreign samples representative of the subspecies dalmatica, pallasiana, laricio, nigra and salzmannii. The cumulative percentage of total variation explained by the first three coordinates was 74.50%.