Single-nucleus transcriptional profiling of chronic kidney disease after cisplatin nephrotoxicity

Cisplatin induces both acute and chronic nephrotoxicity during chemotherapy in cancer patients. Here, we report the study of single-nucleus sequencing (snRNA-seq) of cisplatin-induced nephrotoxicity. Male C57BL/6 mice were given 4 consecutive weekly injections of 8 mg/kg cisplatin. Kidney tissues were collected at 9 weeks after the first cisplatin injection for single-nucleus RNA sequencing (snRNA-seq)