Transcriptomic profiling of senescent human stromal cells upon medicinal intervention with rutin

Aging and age-related pathologies can be delayed by specifically targeting the senescence-associated secretory phenotype (SASP), a hallmark feature of senescent cells. Achieving the goal using natural or synthetic agents would have a tremendous impact on the quality of lifespan and burden of age-related chronic diseases. We report the potential of rutin, a bioactive phytochemical component derived from natural plants specifically ginkgo boliva, in targeting senescent cells via suppression of the SASP. This study demonstrates the efficacy of rutin as medicinal agent in controlling the influence of senescent human stromal cells and provides a strong rationale for its future use in aging intervention and geriatric medicine. Overall design: Examination of in vitro efficacy of rutin, a polyphenolic compound in treating human senescent stromal cells (PSC27 line). Ginkgo biloba products are widely consumed herbal supplements that contain ingredients with anti-oxidant potentials. However, the active agents in ginkgo biloba extracts (GBE) remain unclear, and its major ingredients such as rutin has not been extensively investigated in cellular senescence. This assay was performed beyond previous studies reporting the effects of GBE in anti-diabetic, anti-cataract therapy, anti-inflammation and anti-tumor therapies.