In vivo RNA-seq for for Hh Dependent genes in E7.5 mesoderm
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https://www.ncbi.nlm.nih.gov/sra/SRP254885
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Mesp1-Cre+ cells from E7.5 mouse embryos (from the cross Mesp1-Cre/+ x Rosa26-Gli3R-IRES-YFP/tdTomato) were sorted by FACS where wild type (tdTomato-expressing) and mutant (Gli3R + YFP co-expressing) cells were collected separately from single litters. Overall design: Comparing Mesp1Cre sorted E7.5 mesoderm between Wt embryos embryos expressing a dominant negative Hh pathway transcriptional repressor (Gli3R). All 3 samples were litter-matched
创建时间:
2020-05-29



