The effect of siISG15 in OGD-treated microglia

BV2 cells are widely used as cell models for microglia in vitro. The BV2 cells were pretreated with siISG15 or negative control and subjected to oxygen-glucose deprivation and reoxygenation (OGD/R) for two hours, followed by twenty four hours of normal culture. Total RNA was extracted and subjected to high-throughput sequencing.This experiment aims to explore the impact of ISG15 knockdown on gene expression in OGD/R-treated BV2 cells.