Inner filter effect correction for fluorescence measurements in microplates (AddAbs) - experimental data

Experimental data for the paper entitled Reducing the Inner Filter Effect in Microplates by Increasing Absorbance? Linear Fluorescence in Highly Concentrated Fluorophore Solutions in the Presence of an Added Absorber (https://doi.org/10.1021/acs.analchem.3c01295).

Separate worksheets are provided for the following:

1. Fluorescence measurements - raw values in triplicate; 2 worksheets for transparent (T) and nontransparent (NT) microplates, each with 15 titrations: L1-L12 and H1-H3,

2. Absorbance measurements - raw values in triplicate (λex = 345 nm, λem = 390 nm); 1 worksheet for T microplates only, with 4 titrations: L1-L4,

3. Fluorescence measurements - averaged, baseline-corrected averaged and normalized baseline-corrected averaged triplicates; 2 worksheets for T and NT microplates, each with 15 titrations: L1-L12 and H1-H3,

4. ZINFE/NINFE-corrected fluorescence in T microplates; 12 worksheets for 12 titrations: L1-L9 and H1-H3,

5. ZINFE/NINFE-corrected fluorescence in NT microplates; 12 worksheets for 12 titrations: L1-L9 and H1-H3.

The results of the ZINFE and NINFE correction methods obtained using the online calculator service written in Javascript: https://ninfe.science (version 09.5.2022.)

For additional details please visit: https://glymech.pharma.hr//GlyMech.html.