Effects of alcohol and PARP inhibition on RNA ribosomal engagement in cortical excitatory neurons. Effects of alcohol and PARP inhibition on RNA ribosomal engagement in cortical excitatory neurons

The objective of the study is to describe the effects of EtOH on excitatory pre-frontal cortical neuronal ribosomal-engagement (RE) transcripts from total-RNA and provides insights into Poly (ADP-ribose) polymerase (PARP)-mediated regulation of EtOH effects (using the PARP inhibitor ABT-888). Overall design: Mice whose ribosomes were tagged with EGFP expressed only in CaMKIIα expressing cells were used for the study. PFC samples were homogenized in the appropriate buffer and TRAP-RNA (collected by anti-EGFP antibody incubation) and total-RNA samples were extracted. Following quantification and quality control, the samples were subjected to rRNA depletion, library preparation and 50bp paired-end sequencing (NovaSeq 6000) using the Illumina platform. Data were analyzed using standardized bioinformatic packages and normalized based on treatment status, i.e., Control, EtOH or EtOH+ABT-888, as well as by sample type, i.e., Input or TRAP.