Single Cell sequencing of E14.5, E15.5 and E14.5 Kir electroporated cortical neurons.

Experimental in vivo hyperpolarization of E14.5 apical progenitors shifted their transcriptional programs with precocious generation of intermediate progenitors, and a forward shift in the laminar, molecular, morphological and circuit features of their neuronal progeny. In particular, we reported an overall repression of L4-type neuronal genes and a premature induction of L2/3-type neuronal genes following Kir2.1 overexpression. Mice were electroporated with pCAG-Kir2.1- IRES-GFP at E14.5 or with pCAG-IRES-GFP at E15.5.FlashTag was injected in these pups after 12h to compare isochronic cohorts of labeled cells within single animals (see Telley et al.,DOI: 10.1126/science.aad8361).