20240305 Gray Figure 3 data

FIG 3 Copper, zinc, and nickel inhibit RclA activity in vitro. Reactions (500 µl) contained 10 mM HEPES-KOH buffer (pH 7.4), 160 µM NADH, 100 µM HOSCN, and the indicated concentrations of metal salts, and were started by addition of 10 nM RclA. I quantified NADH consumption at A340 for 1 min at 20°C and calculated specific activities as µmol NADH consumed min-1 mg-1 RclA (n=3-4 experimental replicates; error bars of 1 standard deviation). Asterisks indicate activities significantly different from that of RclA with no metals added in a given set of experiments (one-way ANOVA with Dunnett’s multiple comparisons test; ** = P<0.01. *** = P<0.001, **** = P<0.0001). X symbols indicate that RclA precipitated at 100 µM CuCl2.