Comparison of bioprinted kidney organoids in different conformations

Bulk RNA-seq comparison of kidney organoids bioprinted in 3 different conformations with varying starting cell densities. Density is dictated by the ratio of bioprinter tip movement to the amount of extrusion, where higher ratios spread cells over a larger surface area. We compare organoids printed with no movement ('blob', ratio 0) to those with moderate ('line 3', ratio 20) or high movement ('line 1', ratio 40). Overall design: Each condition consistes of 3 replicate organoids, where each replicate was generated from a distinct well of differentiated iPSCs.