Effect of Holstein genotype on immune response to an intramammary Escherichia coli challenge

Since 1964, the University of Minnesota has selectively bred a Holstein herd to maintain genetically static, unselected Holsteins (UH). Comparison of these UH cows with contemporary Holstein (CH) has demonstrated that the UH cows not only produce less milk but also have fewer health concerns than their CH herdmates. The objective of this study was to determine the effects of Holstein genotype on innate immune response in an experimental intramammary E. coli challenge model. Primiparous UH (n= 5) and CH (n = 7) cows received 430 cfu E. coli strain P4 in 1 quarter. Blood samples were collected at 0, 0.5, 1, 2, 7d relative to E. coli infusion. RNA Sequencing was conducted using the HiSeq 2500 platform (Illumina, San Diego, CA) to generate an average of 46.96 ± 2.67 million 100 bp paired end reads per sample.

For more information about this dataset contact:
John Lippolis: [e-mail]
Hao Ma: [e-mail]

Resources in this dataset:
Resource Title: Effect of Holstein genotype on immune response to an intramammary Escherichia coli challenge.
File Name: Supplemental Table 1.csv
Resource Description: The results of RNA sequencing analysis. The raw sequencing data were submitted to NCBI SRA database with reference PRJNA808034.