MOESM5 of Gene expression differences between PAXgene and Tempus blood RNA tubes are highly reproducible between independent samples and biobanks

Additional file 5. Flowchart of the modified protocol. The modified protocol consists of three parts: (A) collecting blood, (B) processing stabilized blood, and (C) isolating RNA. The overview of the protocol is outlined in the first column (“Process”), and the reagents for each step is given in the second column (“Reagents”). The modified protocol is assembled from three kits: Tempus (blue), PAXgene (cerise), and mirVana (green); note that the reagents used from the Tempus kit are universal and can be replaced with equivalent reagents from other suppliers.