Re-expression of SMARCA4/BRG1 in Small Cell Carcinoma of Ovary, Hypercalcemic Type (SCCOHT) promotes an epithelial-like gene signature through an AP-1-dependent mechanism [A427_RNA-seq]

A427 (NSCLC - Non-Small Cell Lung Cancer) cells +/- BRG1 re-expression - RNA-seq Overall design: AA427 pINDUCER20-BRG1 cell lines were generated by lentiviral infection of the pINDUCER20-BRG1 construct (Gateway Cloning) and subsequently selected in G418 containing medium +selecting in 400 µg/ml G418 to create a mass population. Following selection, the mass population was single cell cloned and screened for BRG1 expression after DOX (1 µg/µl) induction for 48 hours by western blotting. Clones that were able to induce BRG1 were pooled to create a mass pool. A427 pIND20-BRG1 cells were induced with DOX (1 µg/µl) for 48 hours and cell pellets were harvested for RNA-seq.