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CODEX multiplexed imaging cell datasets used for using STELLAR to transfer cell type annotations to other tissues and donors

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NIAID Data Ecosystem2026-03-13 收录
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http://datadryad.org/dataset/doi%253A10.5061%252Fdryad.g4f4qrfrc
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We performed CODEX (co-detection by indexing) multiplexed imaging on 24 sections of the human intestine from 3 donors (B004, B005, B006) using a panel of 47 oligonucleotide-barcoded antibodies. We also performed CODEX imaging on both human tonsil and Barrett's esophagus (BE) using a panel of 57 oligonucleotide-barcoded antibodies.  Subsequently images underwent standard CODEX image processing (tile stitching, drift compensation, cycle concatenation, background subtraction, deconvolution, and determination of best focal plane), single cell segmentation, and column marker z-normalization by tissue. Output of this process were dataframes of 870,000 cells and 220,000 cells respectively with fluorescence values quantified from each marker. Methods See README file.
创建时间:
2022-07-19
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