Allozyme data from Tasmanian galaxiids

Allozyme electrophoresis on muscle homogenates was undertaken as described elsewhere (Richardson et al. 1986; Hammer et al. 2007). Homogenates were successfully screened for various combinations of the enzymes employed in our other studies on galaxiids (Adams et al. 2014; Morgan et al. 2016). Histochemical stain recipes plus enzyme, locus and allozyme nomenclature all follow Hammer et al. (2007). Adams M, Raadik TA, Burridge CP, Georges A (2014) Global biodiversity assessment and hyper-cryptic species complexes: More than one species of elephant in the room? Systematic Biology 63, 518–533. Hammer MP, Adams M, Unmack PJ, Walker KF (2007) A rethink on Retropinna: conservation implications of new taxa and significant genetic sub-structure in Australian smelts (Pisces : Retropinnidae). Marine and Freshwater Research 58, 327–341. Morgan DL, Beatty SJ, Close PG, Allen MG, Unmack PJ, Hammer MP, Adams M (2016) Resolving the taxonomy, range and ecology of biogeographically isolated and critically endangered populations of an Australian freshwater galaxiid, Galaxias truttaceus. Pacific Conservation Biology 22, 350–359. Richardson BJ, Baverstock PR, Adams MA (1986) ‘Allozyme electrophoresis. a handbook for animal systematics and population studies.’ (Academic Press: Sydney, NSW, Australia