Studies of eRF1 in mammalian cells
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https://www.ncbi.nlm.nih.gov/sra/SRP500858
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We performed ribosome profiles in the eRF1 depleted cells under the wild-type backgound and GCN2 knockout background in mouse embryonic stem cells Overall design: After RNase1 digestion of cell lysate, 80S ribosomes were isolated by sucrose density gradient. Then the ribosome protected fragments were isolated, followed by library constructions for high throughput sequencing.
创建时间：
2025-10-25
