Immunoblot and RPPA analysis of the senescence-associated proteins p21 and members of PI3K/Akt/mTOR pathway.

A representative immunoblot of p21 expression levels at different time points and dose rates is shown (A). The columns represent protein levels of p21 (B) Akt (C), phospho-Akt (D), PI3K (E) and mTOR (F) in control (blue), 1.4 mGy/h (orange) and 2.4 mGy/h (green) irradiated HUVECs. The average ratios of relative protein expression in control and irradiated samples are shown. The protein bands were quantified using TotalLab TL100 software by integration of all the pixel values in the band area after background correction and normalized to the actin expression. The data are represented as ± SEM. Three biological replicates were used in all experiments. (Students t-test; *p