Mobile fractions and halftimes from FRAP analysis of GFP, GFP-SOD1WT or GFP-SOD1G93A in WT motor neurons.

15 wildtype motor neurons expressing either GFP, GFP-SOD1WT or GFP-SOD1G93A were studied using FRAP and the FRAP recovery curves recorded. Kinetic Image analysis software was used to fit a halftime of recovery to the FRAP curves and to calculate a mobile fraction.The halftime values of the main 50µm ROI were plotted on a Q-Q plot – comparing observed and expected valueswhich demonstrated the data were not distributed normally.. To test whether there was a difference between halftime values of wildtype neurons expressing GFP, GFP-SOD1WT or GFP-SOD1G93A, a Kruskal-Wallis test was applied. This test is a non-parametric means of comparing groups of results. A significance value of less than 0.05 implies a statistically significant difference between the groups studied. The results of this analysis are for the Mobile fraction: Chi-square value = 5.180; Degrees of Freedom = 2; Significance level = 0.075. As the significance level is greater than 0.05, this demonstrates that the mobile fractions of GFP, GFP-SOD1WT or GFP-SOD1G93A have not been shown to be different under the test conditions used.