The cellular response to the deprivation of L-phenylalanine from the medium in the cultivation of PANC-1, a pancreas carcinoma cell line.

To optimize the efficacy of boron neutron capture therapy (BNCT), we investigated the optimal conditions for maximizing the uptake of boronophenylalanine (BPA), an analog of L-phenylalanine (L-Phe), in tumor cells. In the cultivation of PANC-1 cells, an amplified accumulation of BPA was observed under 24 hours of L-Phe deprivation, which resulted in an enhancement of BNCT efficiency. Consequently, we conducted a comprehensive analysis of the cellular response of PANC-1 under L-Phe restriction by RNA-seq.