Bulked segregant analysis (BSA) of slrx620 (suppressor of lrx345-620) mutant based on genome sequencing

To identify cellular components in the LRX3/4/5-RALFs-FER salt stress response pathway in Arabidopsis, we performed ethylmethane sulfonate (EMS)-mediated mutagenesis in the lrx345 mutant and screened for suppressors that exhibited a higher survival rate than the lrx345 mutant under salt stress. Here, we report one of these suppressors, slrx620 (suppressor of lrx345-620), which exhibited a longer petiole and a higher survival rate under salt stress than the lrx345 mutant. To identify the mutated gene in the slrx620 that was responsible for the suppression of the lrx345 mutant phenotypes, bulked segregant analysis (BSA) was conducted.