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Next Generation Sequencing Facilitates Transcriptomes Quantitative Analysis of N2A cells transfected with the plasmids respectively encoding RfxCas13d/dRfxCas13d and crRNA.

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NIAID Data Ecosystem2026-03-14 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE222461
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To determine what effect the collateral activity of RfxCas13d has on N2acells. The plasmids encoding RfxCas13d/dRfxCas13d and crRNA were transfected into N2a cells. After 48 hours of transfection, GFP-positive cells were collected using FCAS and extracted for total RNA. Then, we analyzed the transcriptome of each group with RNA-seq and analyzed the differential genes among the groups. mRNA profiles of N2A cells transfected with the plasmids respectively encoding RfxCas13d/dRfxCas13d and crRNA.
创建时间:
2023-01-14
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