Repeatability and reproducibility of the real-time PCR assay.

Note:
a all the DNA samples were extracted with one individual mite with different extraction methods, QG = Qiagen DNeasy Blood and Tissue kit; ZR = ZR Tissue & Insect DNA kit (Zymo Research, CA, USA); KF = Kingfisher Cell and Tissue kit (Thermo Scientific, USA).
b Repeatability is calculated as the percent coefficient of variance (%CV) of Cqs of a sample within a single run.
c Reproducibility is calculated as the percent coefficient of variance of Cqs of a sample across independent runs.
Repeatability and reproducibility of the real-time PCR assay.