Survival and bacterial clearance at later timepoints in Ifnar−/− and wildtype animals following sequential poly I:C treatment and S. pneumoniae pulmonary infection.

A. Age- and sex-matched wildtype (WT) and Ifnar−/− animals were administered i.n. poly I:C for 3 days, followed by i.t. S. pneumoniae (1×104 CFU). Animals were monitored for 14 days after pneumococcal infections for indications for euthanasia due to premoribund state. **, p = 0.001 by Log-rank test, poly I:C-treated Ifnar−/− vs. poly I:C-treated WT group; n = 7–8/group. All other comparisons were not significant. Data is representative of 2 separate experiments. B, C. In separate experiments, age- and sex-matched wildtype (WT) and Ifnar−/− animals were administered i.n. poly I:C for 3 days, followed by i.t. S. pneumoniae (1.5×104 CFU). On day 3 and 4 following bacterial infection, animals were euthanized for harvest of lungs (B) and collection of blood (C) samples, followed by enumeration of bacterial CFU. *, p