656d00d5-0e2e-44f3-b6ce-e8787de9b107 - samples
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下载链接：
https://www.omicsdi.org/dataset/ega/EGAD00001008674
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资源简介：
To investigate the mode of action and potential side-effects we analyzed differential gene expression in
Postmitotic C9orf72 iPSC-Neurons by RNAseq. The cells were treated in a 2 dose regime at 10 µm in 0.1 % DMSO for 9 days. Compound treated iPSC-Neurons were washed with PBS, frozen on dry ice and stored at -80°C until RNA
isolation. The RNA was isolated using miRNA Mini Kit (Qiagen) using 700 μl of Qiazol. A total of 250 ng of
RNA per sample was processed for mRNA library preparation as per the manufacturer’s instructions for
Illumina® Stranded mRNA Prep Ligation to be used with the IDT® for Illumina® RNA UD Indexes Set B and sequenced using NextSeq 500/550 High Output Kit v2.5 (Illumina) on NextSeq 550 (Illumina). The data
was processed and analyzed using CLC genomics workbench (Version 21.0.3, Qiagen)EGA dataset EGAD00001008674
创建时间：
2022-06-08
