Nivolumab to restore T-cell fitness in CAR-T refractory multiple myeloma

We here applied single-cell RNA sequencing of circulating T-cells from primary MM patients to perform transcriptional profiling and assess T-cell fitness in the context of emerging resistance to CAR-T cell therapy. Response to PD-1 inhibition after CAR-T was dictated by the fitness state of non-CAR T cells A total of 1,047 single cells from four patients before and after checkpoint inhibitor salvage treatment were examined by full-length single-cell RNA sequencing. Quality filtering resulting in the retention of 1,007 single cells. Due to consistency with available pre-and post timepoints the heatmap shown in Figure 1E was generated only for MM1 and MM2 with a total of 164 retained CD3+ T-cells. ***Raw data not submitted to GEO due to patient privacy concerns.*** *************************************************************** Raw files for human/patient samples were not submitted to GEO due to concerns about submitting personally identifiable sequence data for open access. ***************************************************************