Toll-like receptor 4 signaling in osteoblasts is required for load-induced bone formation in mice

In this study, we aimed to determine the function of toll-like receptor 4 (TLR4), a key receptor in the NF-κB signaling pathway expressed in osteoblasts. Specifically, we hypothesized that the loss of TLR4 signaling in osteoblasts would result in diminished load-induced bone formation due to decreased NGF expression following loading. RNA sequencing from loaded and non-loaded bones from wildtype and CKO mice