Specific binding of validated ORFs to c-di-GMP.

1 Binding and enzymatic activity is deemed positive if activity has been demonstrated for the purified protein or if mutation of residues required for c-di-GMP binding or enyzmatic activity regulate a c-di-GMP dependent phenotype.(+) = c-di-GMP binding observed,DGC = diguanylate cyclase activity observed,PDE = phosphodiesterase activity observed.Two-way ANOVA was used to determine if the fraction bound to 32P-c-di-GMP in the presence unlabeled c-di-GMP competitor differed significantly from no competitor (NC).* = p Specific binding of validated ORFs to c-di-GMP.