Effect of Aloin on cell viability of human foreskin fibroblast Hs68 cells exposed to 43°Cwith/without Aloin supplement.

(A) The optical microscopy images (magnification, ×100) of fibroblast cell and (B) diagram of curve of cell viabilities were representative of three independent experiments. Cells were exposed to heat stress with a single administration of Aloin (43°C+A150) or (43°C+A300), vehicle (43°C), or were left unheated and given the vehicle (CT). The cell viabilities were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The data are expressed as the means ± SEM of two replicates of three samples (n = 6). The asterisks indicate a significant difference (* P<0.05, ** P<0.01, *** P<0.005) compared to the control group; the crosses indicate a significant difference (++ P<0.01, +++ P<0.005) compared to the heat-treated group.