Transcriptome of cells from draining lymph nodes of mice during experimental autoimmune encephalomyelitis (EAE)

Cells were prepared from draining lymph nodes (dLN) from naïve C57BL6/N female mice, as well as from C57BL6/N female mice at day 1 and day 3 after subcutaneous immunization with MOG(35-55) emulsified in Complete Freund’s adjuvant (CFA). Some mice received the CpG-B-1826 (50 microg; TCCATgACgTTCCTgACgTT; TIB MOLBIOL Syntheselabor GmbH), which was added to the MOG(35-55)/CFA mixture before emulsification. Cells from dLN were directly lysed in 350 microlitres RLT buffer containing 1% beta-mercaptoethanol (Invitrogen). Mice were 6-10 weeks of age at the time of immunization. The goal was to identify genes differentially expressed between dLN cells from mice immunized with the classical EAE protocol versus mice immunized with the addition of CpG-B-1826 to the immunization cocktail in order to gain some insights into the immune-modulatory effect of this treatment using an unbiased approach. The experiments include a total of 15 arrays, each corresponding to a distinct biological sample. There are 3 arrays (3 samples) for dLN cells from naïve mice. There are 3 arrays (3 samples) for dLN cells taken from mice on day 1 post-immunization using the classical “control” protocol. There are 3 arrays (3 samples) for dLN cells taken from mice on day 3 post-immunization using the classical “control” protocol. There are 3 arrays (3 samples) for dLN cells taken from mice on day 1 post-immunization using the protocol supplemented by the addition of CpG-B-1826 to the immunization cocktail. There are 3 arrays (3 samples) for dLN cells taken from mice on day 3 post-immunization using the protocol supplemented by the addition of CpG-B-1826 to the immunization cocktail.