Arabidopsis thaliana Raw sequence reads

Our genetic and biochemical analyses indicated that ARF6 and ARF8 are downstream of SPY in regulating parthenocarpy. To identify ARF6, ARF8 and SPY responsive genes that are involved in fruit set and growth, transcriptome analysis was performed by RNA-seq using the following samples, unpollinated pistils at 2 days before anthesis, from stage 10 flowers of arf6-2, arf8-3, spy-3 and WT; and 0 DAA WT pistils from stage 14 flowers that were already self-pollinated. Two biological repeats were included in each set of samples. Total RNAs were purified from unpollinated pistils from stage 10 flowers of arf6-2, arf8-3, spy-3 and WT, and pollinated pistils from WT stage 14 flowers (0 DAA). RNA-Seq cDNA libraries, two biological repeats, were prepared with the Quant Seq mRNA-Seq library prep kit FWD for Illumina.