Non-Canonical Hedgehog Signaling Mediates Profibrotic Hematopoiesis-Stroma Crosstalk in Myeloproliferative Neoplasms

We sorted lineage negative hematopoietic cells from mice previously transplanted with WT or Gli1 knockout bone marrow in both control and fibrosis settings. Cells were via magnetic depletion and facs sorting and performed scRNAseq on them. We identified 13 hematopoetic cells clusters across all lineages and one non-hematopoietic stromal cell cluster Overall design: We used scRNA 3' RNA sequencing product from 10x Genomics to investigate the role of hematopoietic Gli1 in Jak2V617F-induced myelofibrosis