Mouse Dbh-/- vs. Dbh+/+ E10.5 hearts

Genomic microarray analysis of adrenergic-deficient (Dbh-/-) vs. wild-type control (Dbh+/+) mouse heart expression at embryonic day 10.5 (E10.5). Four Dbh-/- and four Dbh+/+ E10.5 hearts were isolated, and RNA was extracted from each for genomic microarray analysis using Affymetrix 430A 2.0 arrays. The biological samples were collected and prepared in Dr. Ebert's laboratory at the Univ of Central Florida in Orlando, FL. The RNA samples were sent to GeneLogic (Gaithersburg, MD) for the microarray analysis.本数据集针对胚胎第10.5天（E10.5）的肾上腺素能缺陷型（Dbh-/-）与野生型对照（Dbh+/+）小鼠心脏的基因表达开展基因组微阵列分析。本研究共分离得到4例Dbh-/-型与4例Dbh+/+型E10.5小鼠心脏，分别提取其总RNA，采用Affymetrix 430A 2.0微阵列芯片完成基因组微阵列分析。生物学样本的采集与制备工作均于美国佛罗里达州奥兰多市中佛罗里达大学Ebert博士的实验室内完成，所有RNA样本随后被送往位于马里兰州盖瑟斯堡的GeneLogic公司进行微阵列分析。