Data underlying the chapter and publication: Synthesis of Chiral Amines via a Bi-Enzymatic Cascade Using an Ene-Reductase and Amine Dehydrogenase

This dataset contains data collected during experiments at Delft University of Technology, as a chapter 2 of the dissertation written by Ewald Jongkind and the corresponding publication. The aim of this work was to produce chiral amines by using two enzymes in a one-pot cascade: the Old Yellow Enzyme (OYE) and Amine Dehydrogenase (AmDH). Dataset includes protein purification gels images, calculated enzyme concentrations, calculated conversions by the enzymes and enzymatic activities in Excel. More details are found in the publication and the README-file

本数据集涵盖埃瓦尔德·容金德（Ewald Jongkind）所撰学位论文第二章及其对应发表论文中，于代尔夫特理工大学（Delft University of Technology）实验期间采集的相关数据。本研究的目标为通过一锅法级联反应联用两种酶制备手性胺：老旧黄色酶（Old Yellow Enzyme, OYE）与胺脱氢酶（Amine Dehydrogenase, AmDH）。数据集包含以Excel格式存储的蛋白质纯化凝胶图像、酶浓度计算值、酶催化转化率计算结果以及酶活性数据。更多详细信息可参阅对应发表论文与README文件。