Gene expression analysis of developing limbs

To evaluate gene expression profile in developing joints vs adjacent growth plate in control and TGF-beta type II receptor conditional knock-out in limb mesenchyme. We obtained E14.5 autopods from two normal controls and two Tgfbr2Prx1KO littermate embryos. Samples were frozen in OTC and cryosectioned and subjected to laser-capture microdissection for RNA sampling for interzone, growth plate chondrocytes and interdigital tissue. RNas were subjected to microarray analysis本研究旨在对比对照组与肢间充质中转化生长因子βⅡ型受体（TGF-β type Ⅱ receptor）条件性敲除模型中，发育中关节与其邻近生长板的基因表达谱差异。我们从2只正常对照胚胎及2只Tgfbr2Prx1KO同窝胚胎中获取了胚胎发育第14.5天（E14.5）时期的肢远端组织。样本经冰冻切片包埋剂（OTC）包埋后进行冰冻切片，随后通过激光捕获显微切割分别采集关节间区、生长板软骨细胞及趾间组织的RNA样本。对所获取的RNA样本开展基因微阵列（microarray）分析。