Pilosella piloselloides subsp. praealta strain:R35 Transcriptome or Gene expression. Pilosella piloselloides subsp. praealta strain:R35

Hieracium praealtum forms seeds asexually by apomixis. During ovule development, the sexual pathway ceases when a diploid aposporous initial (AI) cell differentiates, enlarges and undergoes mitosis forming an aposporous embryo (AE) sac that displaces sexual structures. This study characterises the transcriptome of three ovule cell types, aposporous initial (AI), early aposporous embryo (EAE) sac and somatic ovule (SO) cells from apomictic Hieracium praealtum. Laser capture microdissection was used to isolate specific ovule cell types during early aposporous embryo sac formation. Amplified message RNA was sequenced using 454 pyrosequencing. This study provides a high-resolution characterisation of gene expression in cell types highly relevant to the asexual pathway.