RNA-seq of ventricle and fat tissues of male and female cardiac myocyte-specific estrogen receptor KO mice

Cardiac myocyte-specific ERalpha KO mice were generated to assess the role of ERalpha in the heart. Female ERalphaHKO mice displayed a modest cardiac phenotype, but unexpectedly, an the most striking obesity phenotype developed was obesity in female ERalphaHKO but not male ERalphaHKO mice. In female ERalphaHKO mice we identified cardiac dysfunction, mild glucose and insulin intolerance, and reduced ERalpha gene expression in skeletal muscle and white adipose tissue (WAT). RNA-seq analysis was conducted on the ventricles and WAT of male and female ERalphaHKO mice to further elucidate the transcriptomic alterations associated with the shift in metabolic profiles in the tissues of interest.