Lethal vulva bursting phenotype observed in different mutant backgrounds.

The indicated strains (grown at 15°C or 20°C, as indicated) were scored for bursting through the vulva at the developmental transition to the adult stage. The percentage of bursted animals is shown. The number of animals scored (n) is indicated. The value for let-7(n2853) vps-52(qbc4) was determined from individually genotyped progeny of let-7(n2853) vps-52(qbc4)/+ mothers. The same procedure was used for let-7(n2853); vps-53(ok2864) mutant. Animals were fed bacteria expressing either control (control(RNAi)) or alg-1 targeting (alg-1(RNAi)) dsRNA, as indicated.