遇见数据集

Catalysis of MUF-triNAG by hen egg-white lysozyme (dataset formatted for analysis by interferENZY)

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Zenodo2020-07-27 更新2026-05-25 收录
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<strong>Main description</strong> This dataset depicts the catalysis of the fluorogenic substrate MUF-triNAG to the fluorescent substrate MUF by hen egg-white lysozyme, for fixed concentration of enzyme and variation of concentration of initial substrate. It was originally documented in <em>Phys. Chem. Chem. Phys., 2020, 22, 16143-16149</em> (https://doi.org/10.1039/D0CP02469D) and then used as a study case for the webserver interferENZY (a web-based tool for enzymatic assay validation and standardized kinetic analysis; visit https://interferenzy.i3s.up.pt for more information). To this end, it was converted to the format here presented: tab-separated *.txt input required for interferENZY analysis. <strong>Dataset organization</strong> Line 1: Tab-separated initial concentrations of substrate MUF-triNAG in micromolar (µM) concentration Line 2: Concentration of enzyme (0.25 µM) Line 3: Units of time Line 4: Units of concentration for substrate values and measurements Line 5: Dataset name Line 6 and downwards: Tab-separated column-pairs of the progress curves (time,Product) corresponding to the indicated values of initial concentrations of substrate in line 1 <strong>Contact information:</strong> Maria Filipa Pinto (mfpinto@i3s.up.pt)<br> Pedro M. Martins (pmartins@ibmc.up.pt) i3S – Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal. Telephone number: +351 226 074 900

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Zenodo
创建时间:
2020-07-27
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