The DNA methylation landscape of enhancers in the guinea pig hippocampus

Aim: To determine the state of methylation of DNA molecules that are physically engages with either poised or active enhancers. Methods: We used ChIP-bisulfite-sequencing with H3K4me1 antibody to map the methylation of DNA bound to enhancer. We used ChIP-sequencing with RNApolII-PS5 antibody to map actively transcribing transcription start sites. We used reduced representation bisulfite sequencing (RRBS) to map total DNA methylation. Results - conclusion: DNA molecules that are physically found in H3K4me1 chromatin are hypomethylated while DNA found in enhancers that are associated with active transcription is further demethylated. Overall design: ChIP bisulfite sequencing (H3K4me1 antibody), ChIP sequencing (RNApolII-PS5 antibody) and and RRBS were performed on hippocampus of 3 different wild type 12-week old guinea pig animals.