Self-organizing human neuruloids allow disease modeling in the developing ectodermal compartment
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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http://data.iscr.ac.cn/Article?id=8a0697c4d92ad0954f36145af563e684
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资源简介：
Harnessing the self-organizing abilities of human embryonic stem cells to mimic normal and aberrant development is a pressing challenge. Generation of highly reproducible and standardized models would allow drug screening with unprecedented accuracy. Here we report single-cell RNA-seq data (10xGenomics) of human "neuruloids". Using micro-patterned technologies we recapitulate the self-organization of the complete human embryonic ectodermal compartment during neurulation containing: neural progenitors, neural crest, sensory placode and epidermis. Single-cell transcriptomics allowed the precise molecular characterization of each cell type in a faithful model of human neurulation. Additionally, isogenic neuruloids coupled to deep neural network analysis provide a unique opportunity to model human neuropathological disorders as demonstrated for Huntigntonâs disease.
提供机构：
Rockefeller University
创建时间：
2022-02-20
