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Isolation of nuclei for chromatin analysis in fission yeast.

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PubMed Central1997-11-15 更新2026-05-25 收录
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The methods available for analysis of the chromatin of Schizosaccharomyces pombe are time consuming (>8 h) and/or result in some degradation of the chromatin. Here we report an optimised method for the preparation of spheroplasts and the isolation of nuclei which takes <25 min and is suitable for analysis of chromatin structure by micrococcal nuclease, restriction endonuclease or by immunoprecipitation.

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1997-11-15
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